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The Tree's Knees is the twelfth title in the Looney Tunes series featuring Bosko. It was released as early as May 16, 1931. It was the last cartoon in the series to be directed by both Hugh Harman and Rudolf Ising. Every Looney Tunes cartoon after this was directed by Hugh Harman until 1933, and every Merrie Melodies cartoon was directed by Rudolf Ising until the aforementioned year. It is also the last Bosko cartoon in the Looney Tunes series to not feature the main character's (Bosko's) name in the title. The short is also notable for the extensive use of footage from the earlier short Ain't Nature Grand! that it reuses, in particular a scene of Bosko happily and innocently pursuing a butterfly. The film score was composed by Frank Marsales.