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Set in provincial France sometime in the 1870s, Mam'zelle Nitouche is put in motion by Celestin-Floridor (Raimu), the music master of a convent who secretly doubles as the composer of popular operettas. When our hero sneaks off to the city to witness the premiere of his latest effort, he is followed by convent-bred Nitouche (Janie Marese). Conveniently, the star of the show walks out in a temperamental huff, whereupon Nitouche is pressed into service to sing for the crowd, scoring an enormous hit. The trick now is to sneak back into the convent without alerting the authorities -- a trick that must be pulled off not only by Nitouche but by the sheepish Celestin-Floridor. Tragically, Janie Marese, who so charmingly played the title role in Mam'zelle Nitouche, was killed in an auto accident not long before the film's premiere.