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Experiment Alcatraz stars John Howard as a doctor who develops a serum to cure radioactive poisoning. He tests it on several Alcatraz "lifers," who have volunteered for the experiment on the promise of freedom if they survive. A murder within the group casts aspersions on the doctor, but the mystery is solved before the allotted hour's running time is up. While hardly a surefire candidate for box office success, Experiment Alcatraz was purchased for release by RKO for a sum of $100,000. The film had been independently produced a year before its distribution, its lack of production polish betraying its shoestring origins. Coincidentally, leading man John Howard "continued" in the medical profession as the star of the mid-1950s TV series Dr. Hudson's Secret Journal.